Homologous High-Level Lipase and Single-Cell Protein Production with Engineered Yarrowia lipolytica via Scale-Up Fermentation for Industrial Applications

Yarrowia lipolytica is a promising feed additives. Here, we aimed to produce extracellular lipases and single-cell proteins (SCPs) at high levels simultaneously through fed-batch fermentation of engineered Y. lipolytica. The parameters for 500 mL shake flask cultures were optimized with single factorial design. resultant activity lipase reached 880.6 U/mL after 84 h fermentation, 32.0 g/L broth dry SCP was obtained 120 h. To attain productivity, the high-density scaled up in 10 L, 30 100 L fermentors. Using glycerol as sole carbon source, peaked 8083.3 U/mL, final weight 183.1 94.6 11,100.0 173.3 g SCP/L 136 fermentors, respectively. Following 8532.0 170.3 A balance between secretion growth recombinant strain achieved, indicating that an efficient strategy could promote further scale-up industrial production from